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Three manuscripts from the collections of the Regional Museum and Gallery in Most were digitised in 2020. The oldest of them (shelf mark 1/Ruk) comes from the last third of the 14th century; it comprises the treatise Mariale by the Italian Franciscan Servasanctus of Faenza and a collection of sermons; in the Middle Ages, it was part of the library of the Cistercian monastery in Osek. The second manuscript (shelf mark 201/Ruk) includes records of the guild of tailors in Most from 1568–1828. The last one (shelf mark 26/Ruk) contains the chronicle of the Czech province of the Franciscan Order, which was compiled by Mathias Kollnberger in 1741.

The digitisation of manuscripts from the Royal Canonry of Premonstratensians at Strahov (the Strahov Library) continued in 2020 with another 34 volumes, predominantly deposited under the shelf mark DA III. Most of the codices are of Czech origin and come from the 15th–19th centuries. The oldest manuscript (DA III 24), which was copied by Matěj Čech from Týn nad Vltavou in 1442 and 1444, contains the confessional manual by Thomas of Chobham and the popular theological work of Hugh Ripelin of Strasburg Compendium theologicae veritatis. The collection of Latin sermons by Jan Mystopol with Czech insertions (DA III 16) comes from the second third of the 16th century; sketches of sermons were written by Antonín Jeroným Dvořák of Bor around the middle of the 18th century and complemented slightly later by anonymous drafts of sermons at Strahov (DA III 35); sermons are also written in the binder’s volume DA III 41, with their author mostly being the Strahov Premonstratensian Bohumír Jan Dlabač. Historiographical works are significantly represented, including an autograph of the first part of the work Flori Austrio-Bohemici by Georgius Crugerius in DA III 15, a set of works on the history of the monastery in Kladruby in DA III 14, extracts from the annual reports of Jesuit colleges for 1758–1763 in DA III 32 and DA III 33, extracts on the history of Litoměřice in DA III 38, and handmade copies of the collection of Tomáš Antonín Putzlacher in DA III 44 and DA III 48. The manuscript DA III 36 includes, besides a treatise on the Premonstratensian convent in Dolní Kounice, also an excerpt from the travelogue of the Franciscan Remedius Prutký. Numerous volumes contain notes from lectures: the earliest come from the Jesuit college in Vienna (DA III 5), most of the lectures were held in the 18th and 19th centuries mainly in the Archbishop’s Seminary in Prague and at the Prague university. Other digitised shelf marks include two manuscripts on alchemy (DD V 34 and DG IV 40), the first of which comes from the last quarter of the 16th century and contains i.a. descriptions of experiments associated with the name of Edward Kelly.

The continued digitisation of the codices from the National Library of the Czech Republic mainly focused on the medieval parts of the shelf marks IV and V. In terms of content, they are dominated by theological works; the Biblical exegesis is represented e.g. by commentaries by Nicholas of Lyra (IV.G.17, V.B.19, V.C.6, V.C.14), Robert Holcot (V.B.12) and Haimo of Auxerre (V.C.9, V.C.19); several volumes contain various commentaries on the key theological handbook of the Middle Ages, the Sentences of Peter Lombard (IV.H.21, IV.H.23, V.A.22, V.A.26). The codices further comprise works on philosophy (commentaries on Aristotle’s writings, copied or taught at the Prague university) or those used in pastoral and confessional practice (confessional works, summae confessorum, by Thomas of Chobham and Bartholomew of San Concordio). Natural sciences are the subject of e.g. the book on medicine from the turn of the 14th century that belonged to the master of the Prague university Jan Ondřej called Šindel (meaning ‘Shingle’, IV.F.20), volumes on astronomy (IV.G.10 and V.A.11) and a poem on the medicinal effects of plants Macer Floridus, accompanied by a commentary (IV.G.9). Several volumes include liturgical and preaching texts as well. Historiographic works are represented by the chronicle of an unknown monk of Erfurt (IV.H.25); a manuscript from the monastery in Zlatá Koruna contains a popular collection of hagiographies, Legenda Aurea by Jacobus de Voragine (XIII.C.15). The collection of texts by the Humanist Bohuslaus of Lobkowicz and Hassenstein (I.D.3) comes from the turn of the 16th century. Apart from the manuscripts, the digitised works also comprise five fragments from the 14th and 15th centuries, including i.a. instruments of notaries public, concerning legal and property issues (XXIV.A.57, XXIV.A.226), and fragments of judicial acts of the Prague consistory (XXIV.A.6). A separate group is formed by eight manuscripts from the 17th–19th centuries from the collections of the Slavonic Library. They are mostly written in Church Slavonic, but also in Croatian, Italian and Latin. Besides liturgical and religious texts, they include biographies and writings on heraldry as well.

The National Library of Medicine in Prague digitised six volumes in 2019. A half of them are Czech-written manuscript medical collections or their parts, mostly coming from the second half of the 18th century. They predominantly contain medical recipes, but also texts on bloodletting and on the effects of celestial bodies on human life. Three printed books contain a Latin treatise on arthritis and gout from 1653, the instructions of the archiepiscopal consistory for midwives from 1770 (both printed in Prague), and depictions of surgeries and surgical instruments, printed in Berlin in 1844.