A martyrology from the first half of the 14th century (shelf number M II 100) was digitised from the collections of the Olomouc Research Library in 2025. The manuscript was probably intended for a monastery of the Minorite order and, according to the obituary inscriptions in the margins of the pages, was certainly used in the Czech environment in the Middle Ages, perhaps in Olomouc, where it is evidenced in the 15th century.
The Regional Museum in Litomyšl has made two autographs of Magdalena Dobromila Rettig available online from its collections. The more extensive one (R-207) contains parts of the text Mladá hospodyňka v domácnosti jak sobě počínati má, aby své i manželovy spokojenosti došla, published in Prague in 1840, and documents the author's creative writing style, including corrections and additions to the text (the autograph dates from 1833). The shorter text (R-206) is a German-language autobiography. The manuscript is incomplete or incompletely preserved, yet these notes are the most comprehensive biography of Magdalena Dobromila Rettig.
Four volumes from the Prague Conservatory collections were digitised in 2025. The oldest of these was written in 1718 and contains Alessandro Scarlatti's Il trionfo dell'onore; according to the information on the decorated binding, it was written for Jan Václav of Gallas. The other three date from the late 18th century and are a piano excerpt from Wolfgang Amadeus Mozart's Cosi fan tutte and a cantata by Leopold Koželuh for the coronation of Leopold II.
In 2025, two more manuscripts were made available online from the collections of the Town Museum and Gallery Polička. Under the shelf number K 127 is a copy of the work Zdravá rada lékařská, authored by Jan Tonsoris. The text has been printed repeatedly since 1771 and this copy is probably an adaptation of one of the printed editions. Under the shelf number K 165 are the notes of Tomáš Sýkora, a tailor from Polička, which relate to the years 1666-1794, but the vast majority of them inform about the events of the 18th century.
The Regional Museum in Louny has made five more manuscripts from the last quarter of the 18th to the middle of the 19th century available online from its collections in 2025. Most of them are prayer books (three in Czech and one in German), there is also a copy of a medical work Vysvětlení onich mocných, užitečných a hojitedlných distilovaných olejův (shelf number S 4919).
In 2025, the National Technical Library provided access to seven manuscripts from the last third of the 18th to the second half of the 19th century. The oldest of these is F. J. Höfler's treatise Ob die Erziehung oder der moralische Charakter einer Nation für das erste Grundgesetz aller Staaten angenommen werden könne from 1770 (St 182), the lecture notes of Johann Friedrich August Göttling and Franz Anton Herget, František Josef Gerstner's Bericht über den Zustand der Elbeschiff-Fahrt im Jahre 1822 ( St 428), a report on the London Industrial Exhibition of 1851 and student calculations.
In 2025, the Regional Museum and Gallery in Most digitised six modern manuscripts.Most of them are German-language prayer books from the 18th century, but this classification of contents is not matched by the collection of medical and cooking advice and recipes (118/Ruk) and the collection of sermons, provincial regulations and other documents of the Franciscan Order, compiled in 1743 by the provincial Matthias Kollnberger.
Three manuscripts from the 18th and 19th centuries were digitised from the collections of the Museum and Gallery in Prostějov in 2025. These are copies of the play Čech a Němec aneb Mlejn na hranicích (Br 100) by Jan Nepomuk Štěpánek, and play Jan Dolinský aneb Krevní právo (Br 103) by Emanuel Schikaneder, and a German-language prayer book (Br 119).
The Cosmography of Claudius Ptolemy was digitized from the collection of books of the Latin School in Jáchymov in 2025. The incunabula were printed in Ulm in 1486 by Johann Reger for the publisher Justus de Albano. The edition also contains 32 woodcut map plates, which have been coloured in this copy; the initials have a simple colour decoration. Manuscript marginalia show the use of printing in the 16th century. The book was donated to the Jáchymov library in 1580 by Jiří Hohreuter.
In 2025, the Museum of the Bohemian Paradise in Turnov provided access to a manuscript written in Czech from the mid-18th century (shelf number R 7), which contains mostly cooking recipes, but also medical and veterinary advice or instructions for the preparation of ink.